Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2005
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Haptically Driven Travelling Through Conformational Space
First Joint EUROHAPTICS CONFERENCE and SYMPOSIUM ON HAPTIC INTERFACES FOR VIRTUAL ENVIRONMENT AND TELEOPERATOR SYSTEMS, year: 2005
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High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches
Proteins: Structure, Function, and Bioinformatics, year: 2005, volume: 58, edition: 3
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Hinge-Like Motions in RNA Kink-Turns: The Role of the Second A-minor Motif and Nominally Unpaired Bases
Biophysical Journal, year: 2005, volume: 88, edition: 5
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Characterization of Rhodococcus wratislaviensis strain J3 that degrades 4-nitrocatechol and other nitroaromatic compounds
Antonie van Leeuwenhoek, year: 2005, volume: 87, edition: 1
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Chemometric exploration of an amperometric biosensor array for fast determination of wastewater quality
Biosensors & Bioelectronics, year: 2005, volume: 21, edition: 3
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Indirect quantification of protein modification degree
Year: 2005, type: Conference abstract
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Influence of Ion Parameters On Molecular Dynamics and/or Free Energy Calculation
Workshop on Modelling interactions in Biomolecules II, year: 2005
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Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
Materials and Structure in Chemistry, Biology, Physics and Technology, year: 2005
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Investigation of osteoprotegerin interactions with ligands and antibodies using piezoelectric biosensors
Biosensors & Bioelectronics, year: 2005, volume: 20, edition: 10
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Kinetic constant variability of bacterial oxidation of elemental sulfur
BioMicroWorld-2005, year: 2005