Energy coupling to nitrate uptake into the denitrifying cells of Paracoccus denitrificans
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Biochimica et Biophysica Acta Bioenergetics |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1S-4GTVW4N-1&_user=835458&_handle=V-WA-A-W-AU-MsSAYWW-UUA-U-AABWWUCCCU-AAWEDYZBCU-VAUEDAYAC-AU-U&_fmt=full&_coverDate=09%2F05%2F2005&_rdoc=3&_orig=browse&_srch=%23toc%234898%232005%23982909997%2 |
| Field | Biochemistry |
| Keywords | nitrate transport denitrification bacteria energization ionophores |
| Description | This study deals with the effects of the agents that dissipate the individual components of the proton motive force (short-chain fatty acids, nigericin, and valinomycin) upon the methyl viologen-coupled nitrate reductase activity in intact cells. The data indicate that nitrate transport relies heavily on the pH gradient at neutral pH. A potential dependent step may be of significance in the transporter cycle under alkaline pH. Experiments with sodium-depleted media provided no hints for Na+ as a possible H+ substitute. |
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