Characterization of Rhodococcus wratislaviensis strain J3 that degrades 4-nitrocatechol and other nitroaromatic compounds
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Antonie van Leeuwenhoek |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | 4-nitrocatechol; Rhodococcus; degradation |
| Description | The bacterial strain J3 was isolated from soil by selective enrichment on mineral medium containing 4-nitrocatechol as the sole carbon and energy source. This strain was identified as Rhodococcus sp. on the basis of morphology, biochemical and physiological characterization, determination of a partial sequence of genes for 16S rRNA and FAME analysis. The isolated bacterium could utilize 4-nitrocatechol, 3-nitrophenol and 5-nitroguaiacol as sole carbon and energy sources. Stechiometric release of nitrites was measured during degradation of 4-nitrocatechol both in growth culture and for resting cells. The J3 strain was unable to degrade 4-nitroguaiacol, 2-nitrophenol, 4-nitrophenol, 2,4-dinitrobenzoic acid, 4,5-dimethoxy-2-nitrobenzoic acid and 2,3-difluoro-6-nitrophenol. The J3 strain is deposited in the Czech Collection of Microorganisms as Rhodococcus sp. CCM 4930. |
| Related projects: |