Project information
Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods
- Project Identification
- QD1377
- Project Period
- 6/2001 - 6/2005
- Investor / Pogramme / Project type
-
Ministry of Agriculture of the CR
- Program II
- MU Faculty or unit
-
Faculty of Science
- doc. Ing. Bohuslav Rittich, CSc.
- Keywords
- Bifidobacterium sp.
- Cooperating Organization
-
MILCOM, a.s.
- Responsible person Ing. Petr Roubal, CSc.
Proposed project will be aimed to study 2 important genera - Bifidobacterium and Lactobacillus. These bacteria plae significant role in human nutrition, espetially as source of probiotics and in the possibility of utilisation on dairy industry. The selec ted strains will be study in detail, their biochemical, physiological and morphological characteristics will be described including the optimal conditions of cultivations and preservations. As well as the changes in their features during different usage in milk products will be studied. In many studies it was found that the classification of LAB is unsufficient and does not correspond to the real phylogentic relationships of different species and subspecies. Many molecular genetic methods were develope d in the world during last years and they were used for the characterisation of many industrially important species of bacteria and yeasts. The aim of this project is based on the use of molecular genetics methods (PCR, RFLP, RAPD, plasmid analysis) for
Publications
Total number of publications: 16
2004
-
Využití nosičů s karboxylovými skupinami pro PCR charakterizaci bifidobakterií v mléčných výrobcích
XIII. konference mladých mikrobiologů: Tomáškovy dny, Brno 2-4.6. 2004, year: 2004
2003
-
Hydrophilic particles designed for PCR applications
3rd International Symposium on Separations in BioSciences SBS 2003, 100 Years of Chromatography, year: 2003
-
Identifikace bakterií druhu Bifidobacterium longum pomocí polymerázové řetězové reakce a restrikční analýzy amplifikované ribozomální DNA (ARDRA)
VII. Pracovní setkání biochemiků a molekulárních biologů, year: 2003
-
Identifikace vybraných druhů baktérií mléčného kvašení pomocí polymerázové řetězové reakce
VII. Pracovní setkání biochemiků a molekulárních biologů, year: 2003
2002
-
Identification of Bifidobacterium strains using PCR
Seventh Symposium on Lactic Acid Bacteria, year: 2002
-
Utilisation of nonenzymatic hydrolysis of DNAs for characterisation of extrachromosomal elements in different strains of Bifidobacteria
Seventh Symposium on Lactic Acid Bacteria, year: 2002