Project information
Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods
- Project Identification
- QD1377
- Project Period
- 6/2001 - 6/2005
- Investor / Pogramme / Project type
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Ministry of Agriculture of the CR
- Program II
- MU Faculty or unit
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Faculty of Science
- doc. Ing. Bohuslav Rittich, CSc.
- Keywords
- Bifidobacterium sp.
- Cooperating Organization
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MILCOM, a.s.
- Responsible person Ing. Petr Roubal, CSc.
Proposed project will be aimed to study 2 important genera - Bifidobacterium and Lactobacillus. These bacteria plae significant role in human nutrition, espetially as source of probiotics and in the possibility of utilisation on dairy industry. The selec ted strains will be study in detail, their biochemical, physiological and morphological characteristics will be described including the optimal conditions of cultivations and preservations. As well as the changes in their features during different usage in milk products will be studied. In many studies it was found that the classification of LAB is unsufficient and does not correspond to the real phylogentic relationships of different species and subspecies. Many molecular genetic methods were develope d in the world during last years and they were used for the characterisation of many industrially important species of bacteria and yeasts. The aim of this project is based on the use of molecular genetics methods (PCR, RFLP, RAPD, plasmid analysis) for
Publications
Total number of publications: 16
2005
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Ferrite supports for isolation of DNA from complex samples and polymerase chain reaction amplification
Journal of Chromatography A, year: 2005, volume: 1080, edition: 1
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Magnetic hydrophilic methacrylate-based polymer microspheres for genomic DNA isolation
Journal of Chromatography A, year: 2005, volume: 1064, edition: 1
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RAPD typizace bifidobakterií
IX. Pracovní setkání biochemiků a molekulárních biologů, year: 2005
2004
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Evaluation of magnetic carriers for DNA isolation and polymerase chain reaction
New perspectives of probiotics, year: 2004
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Isolation of genomic DNA using magnetic cobalt ferrite and silica particles
Journal of Chromatography A, year: 2004, volume: 1056, edition: 1-2
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Isolation of PCR-ready DNA from dairy products.
New perspectives of probiotics, year: 2004
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Magnetic hydrophilic methacrylate-based polymer microspheres designed for PCR-ready DNA isolation
Book of Abstarcts, year: 2004
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Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reaction applications
Journal of Chromatography B, year: 2004, volume: 800, edition: 1-2
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Magnetic supports for molecular diagnostics of microorganismus
International Symposium on the Separation of Proteins, Peptides and Polynucleotides, year: 2004
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Testování SPRI metody izolace DNA pro použití v PCR
Česko-Slovenská studentská vědecká konference:Přírodovědecká fakulta Masarykovy univerzity v Brně, 1.5. 2004., year: 2004