Hydrophilic particles designed for PCR applications

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Authors	ŠPANOVÁ Alena SOUDKOVÁ Eva RITTICH Bohuslav HORÁK Daniel
Year of publication	2003
Type	Article in Proceedings
Conference	3rd International Symposium on Separations in BioSciences SBS 2003, 100 Years of Chromatography
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	magnetic microspheres; magnetite; PCR; inhibition
Description	Magnetic hydrophilic nonporous P(HEMA-co-EDMA), P(HEMA-coGMA) and PGMA microspheres were prepared by dispersion (co)polymerization of HEMA and EDMA or GMA in the presence of several kinds of magnetite.It was found that some components used in the preparation of magnetic carriers interfered with PCR. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of non-interferingmagnetic microspheres.
Related projects:	The use of magnetic carriers with immobilized selective ligands in DNA diagnostics Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods