Hydrophilic particles designed for PCR applications

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Authors

ŠPANOVÁ Alena SOUDKOVÁ Eva RITTICH Bohuslav HORÁK Daniel

Year of publication 2003
Type Article in Proceedings
Conference 3rd International Symposium on Separations in BioSciences SBS 2003, 100 Years of Chromatography
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords magnetic microspheres; magnetite; PCR; inhibition
Description Magnetic hydrophilic nonporous P(HEMA-co-EDMA), P(HEMA-coGMA) and PGMA microspheres were prepared by dispersion (co)polymerization of HEMA and EDMA or GMA in the presence of several kinds of magnetite.It was found that some components used in the preparation of magnetic carriers interfered with PCR. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of non-interferingmagnetic microspheres.
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