Magnetic hydrophilic methacrylate-based polymer microspheres for genomic DNA isolation

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Authors	KŘÍŽOVÁ Jana ŠPANOVÁ Alena RITTICH Bohuslav HORÁK Daniel
Year of publication	2005
Type	Article in Periodical
Magazine / Source	Journal of Chromatography A
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	Magnetic and non-magnetic microspheres; carboxyl groups; DNA adsorption
Description	Carboxyl groups containing magnetic and non-magnetic microspheres were used for isolation of genomic DNA. DNA from chicken erythrocytes and DNA isolated from bacterial cellsof Bifidobacterium longum was used for testing of adsorption/desorption properties of magnetic microspheres. The particles were used for isolation of DNA from different dairy products followed by PCR identification of Bifidobacterium strains.
Related projects:	The use of magnetic carriers with immobilized selective ligands in DNA diagnostics Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods