Isolation of PCR-ready DNA from dairy products.

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Title in English	Isolation of PCR-ready DNA from dairy products
Authors	RITTICH Bohuslav ŠPANOVÁ Alena PETROVÁ Kateřina HORÁK Daniel
Year of publication	2004
Type	Article in Proceedings
Conference	New perspectives of probiotics
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	PCR; magnetic particles; dairy products; Bifidobacterium; Lactobacillus
Description	Carboxyl group-containing magnetic nonporous P(HEMA-co-EDMA), P(HEMA-co-GMA)and PGMA microspheres were used for isolation of DNA from dairy products. Different poly(ethylene glycol) (PEG) and sodium chloride concentrations were used to optimize DNA adsorption. Proposed method was used for PCR identification of probiotic Bifidobacterium and Lactobacillus strains in different dairy products.
Related projects:	The use of magnetic carriers with immobilized selective ligands in DNA diagnostics Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods