Isolation of PCR-ready DNA from dairy products.

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Title in English Isolation of PCR-ready DNA from dairy products
Authors

RITTICH Bohuslav ŠPANOVÁ Alena PETROVÁ Kateřina HORÁK Daniel

Year of publication 2004
Type Article in Proceedings
Conference New perspectives of probiotics
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords PCR; magnetic particles; dairy products; Bifidobacterium; Lactobacillus
Description Carboxyl group-containing magnetic nonporous P(HEMA-co-EDMA), P(HEMA-co-GMA)and PGMA microspheres were used for isolation of DNA from dairy products. Different poly(ethylene glycol) (PEG) and sodium chloride concentrations were used to optimize DNA adsorption. Proposed method was used for PCR identification of probiotic Bifidobacterium and Lactobacillus strains in different dairy products.
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