Project information
Transkription activation system for studying the relationship between cytokinin metabolism and action in Arabidopsis and tobacco
- Project Identification
- IAA5004001
- Project Period
- 1/2000 - 1/2002
- Investor / Pogramme / Project type
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Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
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Faculty of Science
- RNDr. Alena Kuderová, CSc.
- Cooperating Organization
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Institute of Biophysics
- Responsible person prof. RNDr. Břetislav Brzobohatý, CSc.
- Responsible person RNDr. Ivana Macháčková, CSc.
Cytokinins (CKs) are known to act in combination with other phytohormones to regulate diverse developmental processes in plants. However, knowledge of molecualr mechanism of CK action is limited. We will use a novel transcription activation system for regulated gene expression to create transgenic plants overproducing individually heterologous enzymes generating free CKs either by glucoside hydrolysis or by de novo synthesis in defined spatial and temporal patterns. We expect that CK release from glucosides will cause. in addition to common effects. several distrinct effects compared to de novo CK synthesis. Analysis of the distinct effects will contribute to understanding of complexity of CK metobolic conversions. This approach will lead to isolation of Arabidopsis lines displaying phenotypes corresponding to defined CK overproduction patterns. In future, the lines will be used, via isolation of suppresor mutations, to isolate genes involved in CK metabolism, and CK signal pathways.
Publications
Total number of publications: 19
2002
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Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine
PHYSIOLOGIA PLANTARUM, year: 2002, volume: 115, edition: 2
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Optimisation of Immunohistochemical Detection of Cytokinins in Transgenic Arabidopsis Seedlings Expressing Activated ipt, a Cytokinin Biosynthesis Gene
Progress in Basic, Applied and Diagnostic Histochemistry, year: 2002
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Phenotype alterations accompanying activation of a cytokinin biosynthesis gene, ipt, during germination and early seedling development in tobacco
Year: 2002, type: Conference abstract
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Tobacco and Arabidopsis seedling development upon activation of ipt, a cytokinin biosynthesis gene.
Year: 2002, type: Conference abstract
2001
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A Novel System for Regulated Expression of Isopentenyl Transferase Gene, ipt, in Arabidopsis Thaliana.
V. Pracovní setkání biochemiků a molekulárních biologů, Sborník příspěvků, Brno 2001, year: 2001
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Anatomical Study of ipt Transgenic Tobacco Embryo and Seedling Meristem Development.
Year: 2001, type: Conference abstract
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Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings
17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts, year: 2001
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Meristem Development in Transgenic Tobacco Seedlings.
Botany in Time of Molecular Biology, Abstracts of 52nd Congress of Polish Botanical Society, year: 2001
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Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.
17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts, year: 2001