Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Description | A novel system allowing a non-invasive manipulation of cytokinin levels is being developed in A. thaliana. Transcriptional fusions between isopentenyl transferase gene, ipt, and a reporter gene, GUS, were inserted behind a synthetic promoter (pOp). pOp is expected to be inactive when introduced into wild type plants but is activated when these plants are crossed with others that express a novel transcription activation protein, LhG4. Transgenic Arabidopsis plants carrying pOp-ipt-GUS and pOp-GUS-ipt were generated by Agrobacterium infiltration technique. The transformed plants developed without any detectable phenotype alteration indicating that pOp promoter is virtually silent in the transformants in the absence of LhG4. The first evidence for activation of ipt and GUS in pOp-ipt-GUS and pOp-GUS-ipt was provided by crossing the individual transformants with an activator line carrying LhG4 under control of CaMV35S promoter (CaMV35S-LhG4). We will report phenotype alterations observed in progeny of crosses of pOp-ipt-GUS and pOp-GUS-ipt with CaMV35S-LhG4. |
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