Molekulární typizace vybraných kmenů bakterií rodu Lactobacillus ze sbírky Laktoflora

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Title in English Molecular typing of selected bacterial strains of genus Lactobacillus from Culture Collection of dairy microorganisms Laktoflora
Authors

DUŠKOVÁ Marta ŠPANOVÁ Alena DRÁB Vladimír RITTICH Bohuslav

Year of publication 2008
Type Article in Periodical
Magazine / Source Mlékařské listy–Zpravodaj
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Lactobacillus; typing; rep-PCR; RAPD
Description Rep-PCR with (GTG)5 primer and RAPD with M13 primer were used for typing of selected strains of genus Lactobacillus collected in Czech Collection of Dairy Microorganisms (CCDM) Laktoflora. Rep-PCR with (GTG)5 primer was more suitable than RAPD with M13 primer. Typing enabled to identified 1 strain into species L. acidophilus and 15 strains in L. helveticus. These results were confirmed with species specific PCRs. They were in accord with biochemical identification in 88% of strains.
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