Molekulární typizace vybraných kmenů bakterií rodu Lactobacillus ze sbírky Laktoflora

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Title in English	Molecular typing of selected bacterial strains of genus Lactobacillus from Culture Collection of dairy microorganisms Laktoflora
Authors	DUŠKOVÁ Marta ŠPANOVÁ Alena DRÁB Vladimír RITTICH Bohuslav
Year of publication	2008
Type	Article in Periodical
Magazine / Source	Mlékařské listy–Zpravodaj
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Lactobacillus; typing; rep-PCR; RAPD
Description	Rep-PCR with (GTG)5 primer and RAPD with M13 primer were used for typing of selected strains of genus Lactobacillus collected in Czech Collection of Dairy Microorganisms (CCDM) Laktoflora. Rep-PCR with (GTG)5 primer was more suitable than RAPD with M13 primer. Typing enabled to identified 1 strain into species L. acidophilus and 15 strains in L. helveticus. These results were confirmed with species specific PCRs. They were in accord with biochemical identification in 88% of strains.
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