Comparison of PCR-ready DNA Isolation Methods from Lactic Acid Bacteria used in Dairy Industry

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Authors	ŠPANOVÁ Alena RITTICH Bohuslav NĚMCOVÁ Eva KŘÍŽOVÁ Jana HORÁK Daniel
Year of publication	2008
Type	Article in Proceedings
Conference	7th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
MU Faculty or unit	Faculty of Science
Citation	ŠPANOVÁ, Alena, Bohuslav RITTICH, Eva NĚMCOVÁ, Jana KŘÍŽOVÁ and Daniel HORÁK. Comparison of PCR-ready DNA Isolation Methods from Lactic Acid Bacteria used in Dairy Industry. In 7th International Conference on the Scientific and Clinical Applications of Magnetic Carriers. Vancouver: University of British Columbia, Canada, 2008, p. 203.
Field	Genetics and molecular biology
Keywords	DNA; isolation; magnetic microspheres; lactic acid bacteria; PCR
Description	Different methods for isolation of PCR-ready DNA were tested. The influence of the DNA isolation procedures tested on the reproducibility of RAPD and rep-PCR was studied. The reproducibility of amplification of DNA isolated by the salting-out method and adsorption on magnetic nonporous microspheres was comparable with the phenol extraction method.
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