Gene expression changes in human prostate carcinoma cells exposed to genotoxic and nongenotoxic aryl hydrocarbon receptor ligands

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Publikace nespadá pod Fakultu sportovních studií, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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HRUBÁ Eva VONDRÁČEK Jan LÍBALOVÁ Helena TOPINKA Jan BRYJA Vítězslav SOUČEK Karel MACHALA Miroslav

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj TOXICOLOGY LETTERS
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1016/j.toxlet.2011.07.011
Obor Genetika a molekulární biologie
Klíčová slova LNCaP cells; benzo[a]pyrene; 2-3-7-8-tetrachlorodibenzo-p-dioxin; cell cycle; WNT5A; E2F1
Popis Carcinogenic polycyclic aromatic hydrocarbons (PAHs) are known as efficient mutagens and ligands of the aryl hydrocarbon receptor (AhR), which has been suggested to play an important role in prostate carcinogenesis. In order to evaluate the complex relationship between the genotoxicity and the AhR-mediated activity of PAHs in prostate cells, we explored global changes in gene expression in LNCaP cells by microarray analysis. We identified 112 genes that were differentially expressed in cells treated for 24 h with BaP, TCDD or both compounds. Our data indicated that the impacts of BaP and TCDD on transcriptome of LNCaP cells significantly overlap, since over 64 % of significantly up-regulated genes and 47% of downregulated genes were similarly affected by both AhR ligands. The AhR ligand-induced Wnt5a upregulation, together with other observed alterations of gene expression, may further contribute to enhanced cell plasticity of prostate carcinoma cells.
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