Gene expression changes in human prostate carcinoma cells exposed to genotoxic and nongenotoxic aryl hydrocarbon receptor ligands
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Article in Periodical |
| Magazine / Source | TOXICOLOGY LETTERS |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1016/j.toxlet.2011.07.011 |
| Field | Genetics and molecular biology |
| Keywords | LNCaP cells; benzo[a]pyrene; 2-3-7-8-tetrachlorodibenzo-p-dioxin; cell cycle; WNT5A; E2F1 |
| Description | Carcinogenic polycyclic aromatic hydrocarbons (PAHs) are known as efficient mutagens and ligands of the aryl hydrocarbon receptor (AhR), which has been suggested to play an important role in prostate carcinogenesis. In order to evaluate the complex relationship between the genotoxicity and the AhR-mediated activity of PAHs in prostate cells, we explored global changes in gene expression in LNCaP cells by microarray analysis. We identified 112 genes that were differentially expressed in cells treated for 24 h with BaP, TCDD or both compounds. Our data indicated that the impacts of BaP and TCDD on transcriptome of LNCaP cells significantly overlap, since over 64 % of significantly up-regulated genes and 47% of downregulated genes were similarly affected by both AhR ligands. The AhR ligand-induced Wnt5a upregulation, together with other observed alterations of gene expression, may further contribute to enhanced cell plasticity of prostate carcinoma cells. |
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