An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	SUMBAL Jakub GUDJONSSON Thorarinn TRAUSTADOTTIR Gunnhildur Asta SUMBALOVÁ KOLEDOVÁ Zuzana
Rok publikování	2022
Druh	Kapitola v knize
Fakulta / Pracoviště MU	Lékařská fakulta
Citace	SUMBAL, Jakub, Thorarinn GUDJONSSON, Gunnhildur Asta TRAUSTADOTTIR a Zuzana SUMBALOVÁ KOLEDOVÁ. An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast. In Vivanco, M.d. (eds) Mammary Stem Cells. Mammary Stem Cells. New York: Springer New York - Humana Press, 2022, s. 283-299. Methods in Molecular Biology, vol. 2471. ISBN 978-1-0716-2192-9. Dostupné z: https://dx.doi.org/10.1007/978-1-0716-2193-6_16.
Popis	Epithelial–stromal interactions play an essential role in regulation of mammary gland development, homeostasis, and tumorigenesis. Fibroblasts constitute a substantial proportion of mammary gland stromal cells in human breast and have been recognized for their paracrine signaling and extracellular matrix production and remodeling roles during normal breast development as well as in breast cancer. However, our current knowledge on human breast fibroblast functions is incomplete. Here we provide a detailed protocol for an organotypic human breast assay to facilitate research in the roles of human breast fibroblasts in mammary epithelial morphogenesis and early tumorigenesis.
Související projekty:	Deciphering the mechanisms of mammary epithelial branched pattern formation through iterative biological and mathematical modelling