STUDY OF VARIOUS PARAMETERS THAT INFLUENCE THE CONTENT OF EXHALED BREATH CONDENSATE USED IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE
| Autoři | |
|---|---|
| Rok publikování | 2018 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY |
| Fakulta / Pracoviště MU | |
| Citace | |
| Doi | http://dx.doi.org/10.1515/hjic-2018-0007 |
| Klíčová slova | capillary electrophoresis; exhaled breath condensate; ionic analysis; pH; gastroesophageal reflux disease |
| Popis | In this work, various parameters that influence the ionic content and pH of exhaled breath condensate in terms of the non-invasive diagnosis of gastroesophageal reflux disease were studied. Exhaled breath condensate samples were collected using a miniature and inexpensive sampling device. Capillary electrophoresis with contactless conductometric detection was used to monitor the ionic content of exhaled breath condensate. Background electrolyte composed of 20 mM of 2-(N-Morpholino)ethanesulfonic acid, 20 mM of L-Histidine, 2 mM of 18-Crown-6 and 30 mu M of cetyltrimethylammonium bromide facilitated the rapid separation of anions and cations, both in less than 2 minutes. The possibility of contamination of the exhaled breath condensate by saliva is discussed in detail. The day-to-day repeatability (n=5) of the ionic content and pH of the exhaled breath condensate was studied and was satisfactory, reflecting mainly the physiological variability. |
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