STUDY OF VARIOUS PARAMETERS THAT INFLUENCE THE CONTENT OF EXHALED BREATH CONDENSATE USED IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

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Authors

GREGUŠ Michal ĎURČ Pavol LAČNÁ Júlia FORET František KUBÁŇ Petr

Year of publication 2018
Type Article in Periodical
Magazine / Source HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY
MU Faculty or unit

Central European Institute of Technology

Citation
Doi http://dx.doi.org/10.1515/hjic-2018-0007
Keywords capillary electrophoresis; exhaled breath condensate; ionic analysis; pH; gastroesophageal reflux disease
Description In this work, various parameters that influence the ionic content and pH of exhaled breath condensate in terms of the non-invasive diagnosis of gastroesophageal reflux disease were studied. Exhaled breath condensate samples were collected using a miniature and inexpensive sampling device. Capillary electrophoresis with contactless conductometric detection was used to monitor the ionic content of exhaled breath condensate. Background electrolyte composed of 20 mM of 2-(N-Morpholino)ethanesulfonic acid, 20 mM of L-Histidine, 2 mM of 18-Crown-6 and 30 mu M of cetyltrimethylammonium bromide facilitated the rapid separation of anions and cations, both in less than 2 minutes. The possibility of contamination of the exhaled breath condensate by saliva is discussed in detail. The day-to-day repeatability (n=5) of the ionic content and pH of the exhaled breath condensate was studied and was satisfactory, reflecting mainly the physiological variability.
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