Impact of Anions, Cations, and pH on Manganese Accumulation and Toxicity in the Green Alga Scenedesmus quadricauda

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	ŠVEC Pavel KOVÁČIK Jozef HEDBAVNÝ Josef BABULA Petr ROTKOVÁ Gabriela KLEJDUS Bořivoj
Rok publikování	2016
Druh	Článek v odborném periodiku
Časopis / Zdroj	Water, Air and Soil Pollution
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Doi	http://dx.doi.org/10.1007/s11270-016-2859-2
Obor	Fyziologie
Klíčová slova	Algae; Antioxidants; Bioaccumulation; Metals; Oxidative stress
Popis	Accumulation of divalent manganese (Mn) and its toxicity in the green alga Scenedesmus quadricauda was studied at circumneutral pH (6.5). A comparison of two applied concentrations (10 or 100 mu M) of MnCl2, MnSO4, and Mn(NO3)(2) indicated that mainly sulfate evoked higher Mn accumulation. On the other hand, nitrate rather depleted antioxidative enzyme activities (APX, CAT, SOD), leading to an increase in ROS formation as proven by fluorescence microscopy. Subsequent experiments revealed that increase in pH (from 4.5 to 9.5) increased also Mn content but typically depleted amounts of reduced glutathione and phytochelatin 2. We also measured the size of particles formed from the manganese salts at pH 9.5. Competitive experiment between Ca/K salts (CaCl2, CaSO4, Ca(NO3)(2), KCl, K2SO4, KNO3) and Mn (as Mn sulfate) showed a negative relation between Ca and Mn amount but KNO3 stimulated Mn accumulation. Microscopy revealed that mainly K salts elevated plasma membrane damage (Acridine orange staining). Data indicate that not only pH but also accompanying anion affects Mn accumulation and that Ca salts may affect Mn toxicity.