Impact of Anions, Cations, and pH on Manganese Accumulation and Toxicity in the Green Alga Scenedesmus quadricauda

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Medicine. Official publication website can be found on muni.cz.
Authors

ŠVEC Pavel KOVÁČIK Jozef HEDBAVNÝ Josef BABULA Petr ROTKOVÁ Gabriela KLEJDUS Bořivoj

Year of publication 2016
Type Article in Periodical
Magazine / Source Water, Air and Soil Pollution
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1007/s11270-016-2859-2
Field Physiology
Keywords Algae; Antioxidants; Bioaccumulation; Metals; Oxidative stress
Description Accumulation of divalent manganese (Mn) and its toxicity in the green alga Scenedesmus quadricauda was studied at circumneutral pH (6.5). A comparison of two applied concentrations (10 or 100 mu M) of MnCl2, MnSO4, and Mn(NO3)(2) indicated that mainly sulfate evoked higher Mn accumulation. On the other hand, nitrate rather depleted antioxidative enzyme activities (APX, CAT, SOD), leading to an increase in ROS formation as proven by fluorescence microscopy. Subsequent experiments revealed that increase in pH (from 4.5 to 9.5) increased also Mn content but typically depleted amounts of reduced glutathione and phytochelatin 2. We also measured the size of particles formed from the manganese salts at pH 9.5. Competitive experiment between Ca/K salts (CaCl2, CaSO4, Ca(NO3)(2), KCl, K2SO4, KNO3) and Mn (as Mn sulfate) showed a negative relation between Ca and Mn amount but KNO3 stimulated Mn accumulation. Microscopy revealed that mainly K salts elevated plasma membrane damage (Acridine orange staining). Data indicate that not only pH but also accompanying anion affects Mn accumulation and that Ca salts may affect Mn toxicity.

You are running an old browser version. We recommend updating your browser to its latest version.

More info