Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction

• Autoři: HUSKA Dalibor; ADAM Vojtech; HUBÁLEK Jaromír; TRNKOVÁ Libuše; ECKSCHLAGER Tomáš; STIBOROVÁ Marie; PROVAZNIK Ivo; KIZEK René
• Rok publikování: 2010
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Chimica Oggi/Chemistry today
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• Obor: Fyzikální chemie a teoretická chemie
• Klíčová slova: ellipticine; DNA; square wave voltammetry; adsorptive transfer technique; automation
• Popis: Manual handling with DNA and drugs can be a source of errors, which can be eliminated also by automation of whole process of sample preparation. The aim of this work was to study interaction between DNA and ellipticine by using of off-line coupled automated pipetting system with adsorptive transfer stripping technique (AdTS) square wave voltammetry (SWV). Interaction between dsDNA and ellipticine was evident from the decrease of CA signal. Six ng of ellipticine per ml resulted in a statistically significant decrease in CA signal (the decrease was higher than the standard deviation of determinations, Student t-test). The decrease in CA signal corresponded to increasing ellipticine concentrations.

Související projekty

• Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni