Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction

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Authors	HUSKA Dalibor ADAM Vojtech HUBÁLEK Jaromír TRNKOVÁ Libuše ECKSCHLAGER Tomáš STIBOROVÁ Marie PROVAZNIK Ivo KIZEK René
Year of publication	2010
Type	Article in Periodical
Magazine / Source	Chimica Oggi/Chemistry today
MU Faculty or unit	Faculty of Science
Citation
Field	Physical chemistry and theoretical chemistry
Keywords	ellipticine; DNA; square wave voltammetry; adsorptive transfer technique; automation
Description	Manual handling with DNA and drugs can be a source of errors, which can be eliminated also by automation of whole process of sample preparation. The aim of this work was to study interaction between DNA and ellipticine by using of off-line coupled automated pipetting system with adsorptive transfer stripping technique (AdTS) square wave voltammetry (SWV). Interaction between dsDNA and ellipticine was evident from the decrease of CA signal. Six ng of ellipticine per ml resulted in a statistically significant decrease in CA signal (the decrease was higher than the standard deviation of determinations, Student t-test). The decrease in CA signal corresponded to increasing ellipticine concentrations.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)