Project information
Analýza klonálních progenitorů plazmatických buněk u monoklonálních gamapatií
- Project Identification
- GAP304/10/1395
- Project Period
- 1/2010 - 12/2014
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Medicine
- Keywords
- Monoclonal gammopathies; plasma cells; progenitors; phenotype; flow cytometry
Detailed study of heterogeneous subpopulations of myeloma cells and their precursors/progenitors in monoclonal gammopathy patients can lead to identification and characterisation of myeloma stem cells. Using polychromatic flow cytometry with sorting of cells, phenotype of myeloma stem cells can be detected and different subpopulation can be selected for clonality assessment. In vitro culturing then can prove self renewal capacity of these myeloma stem cells and their multidrug resistance as well. We suppose that using these approaches we are able to obtain new information in area of pathophysiology of myeloma cells and their behaviour, which can be potentially used for determination of predictive factors applicable in progression of monoclonal gammopaty of undetermined significance to multiple myeloma.
Publications
Total number of publications: 108
2011
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Plasma cell phenotype correlation with cytogenetic and morphological findings in multiple myeloma
Year: 2011, type: Conference abstract
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Polymorphisms Contribution to the Determination of Significant Risk of Specific Toxicities in Multiple Myeloma
Klinická onkologie, year: 2011, volume: 24, edition: Suppl.
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Prognostic significance of morphological assessment of plasma cells in multiple myeloma.
Neoplasma, year: 2011, volume: 58, edition: 6, DOI
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Prognostický význam imunofenotypizace u nemocných s monoklonální gamapatií nejasného významu a mnohočetným myelomem
Klinická biochemie a metabolismus, year: 2011, volume: 19, edition: 2
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Regulatory T Cells Predicts Progression in Previously Untreated Myeloma Patients and Treatment by Cyclophosphamide, Thalidomide Plus Dexamethasone Reduces Regulatory T Cells
Year: 2011, type: Conference abstract
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Sample Processing and Methodological Pitfalls in Multiple Myeloma Research
Klinická onkologie, year: 2011, volume: 24, edition: Suppl.
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Stem cell marker nestin is expressed in plasma cells of multiple myeloma patients
Leukemia Research, year: 2011, volume: 35, edition: 8, DOI
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The necessity of clonality assessment in monoclonal gammopathies
Year: 2011, type: Conference abstract
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Visualization of Numerical Centrosomal Abnormalities by Immunofluorescent Staining
Klinická onkologie, year: 2011, volume: 24, edition: Suppl.
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Vyšetření specifických chromozomových abnormalit u monoklonální gamapatie nejasného významu pomocí interfázní fluorescenční in situ hybridizace
Year: 2011, type: Conference abstract