Project information
Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
- Project Identification
- GV204/97/K084
- Project Period
- 1/1997 - 1/2001
- Investor / Pogramme / Project type
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Czech Science Foundation
- Complex Projects
- MU Faculty or unit
- Faculty of Science
- Cooperating Organization
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Institute of Biophysics
- Responsible person prof. RNDr. Emil Paleček, DrSc.
- Responsible person doc. Ing. Ladislav Novotný, CSc.
- Responsible person RNDr. Bořivoj Vojtěšek, DrSc.
This project of complex studies of biomacromolecule interactions with electrically charged surfaces is based on the recent progress reached in the proposers' laboratories (see the text) in the development of the electrochemical techniques and in the stud ies of interactions of nucleic acids and proteins with the mercury electrode and solid surfaces. In this project basic information about the adsorption/desorption, redox properties and structural features of DNA, RNA, oligonucleotides, proteins and small peptides at interfaces will be obtained. As a result of these studies electrochemical ultramicromethods will be developed including (a) fast response sensors of the DNA damage (b) fast response sensors of the DNA hybridization c new methods for studies of the interaction of DNA with specific proteins and anticancer drugs, etc. In addition information about the DNA local structures in neurological genetic diseases and about the DNA-p53 protein interactions in human cancer will be obtained. The main goal
Publications
Total number of publications: 82
2003
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Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9
Electroanalysis, year: 2003, volume: 19, edition: 15
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Application of elimination voltammetry with linear scan in bioelectrochemistry
The role of electrochemistry in sustained development of modern societies, 54 th ISE Meeting, year: 2003
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Electrochemical detection of chromosomal translocation T (15;17) as an acute promyelotic leukemie marker
Sborník příspěvků, VII. pracovní setkání biochemiků a molekulárních biologů, year: 2003
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Elektrochemické biosenzory v analýze zemědělských produktů a vzorků životního prostředí
CHEMICKÉ LISTY, year: 2003, volume: neuveden, edition: 2003
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Eliminační voltametrie oligonukleotidů
CHEMICKÉ LISTY, year: 2003, volume: 97, edition: 8
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Zobecněný princip eliminační voltametrie
CHEMICKÉ LISTY, year: 2003, volume: 97, edition: 8
2002
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Analytické využití eliminační voltametrie
54. sjezd chemických společností, Brno, year: 2002
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Application of carbon electrodes modified with a mercury layer of a different thickness for studies of the adsorption and kinetics of phase transients of cytidine.
Journal of Electroanalytical Chemistry, year: 2002, volume: 536, edition: 1
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Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping
Bioelectrochemistry, year: 2002, volume: 3, edition: 56
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Electrocatalytic activity of DNA. Electrochemistry in Microscopic and Molecular Dimensions
53-rd Annual Meeting of the International Society of Electrochemistry.Symposium 3: Bioelectrochemistry. Book of Abstracts, year: 2002