Project information
Czech Infrastructure for Integrative Structural Biology
(CIISB)
- Project Identification
- LM2018127
- Project Period
- 1/2020 - 12/2022
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Large Infrastructures for Research, Development and Innovation
- MU Faculty or unit
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Central European Institute of Technology
- prof. RNDr. Vladimír Sklenář, DrSc.
- Cooperating Organization
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Institute of Biotechnology CAS
The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes. CIISB, equipped with the cutting-edge technologies, will offer access to the equipment and expertise in the following fields: high-field NMR spectroscopy; high throughput crystallization of biological macromolecules; X-ray diffraction techniques; Bio-SAXS measurements; AFM imaging; characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, and surface plasmon resonance; high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells; high-end mass spectrometry characterization of biomacromolecules; determination of protein post-translational modifications; MS-based peptide mapping/sequencing; high throughput sequencing and proteomic services. CIISB infrastructure aims to provide a high quality open access services in provision of methods and techniques on a level of national and trans-national importance. CIISB is a part of the ESFRI project INSTRUCT-ERIC.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 327
2021
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Hydrogen-bond directionality and symmetry in [C(O)NH](N)(2)P(O)-based structures: a comparison between X-ray crystallography data and neutron-normalized values, and evaluation of reliability
Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials, year: 2021, volume: 77, edition: 3, DOI
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ICAM-1 induced rearrangements of capsid and genome prime rhinovirus 14 for activation and uncoating
Proceedings of the National Academy of Sciences of the United States of America, year: 2021, volume: 118, edition: 19, DOI
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In vivo and in vitro cell-based model of lung adenocarcinoma from patients with pleural effusion
Neoplasma, year: 2021, volume: 68, edition: 3, DOI
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Insight into formation propensity of pseudocircular DNA G-hairpins
Nucleic acids research, year: 2021, volume: 49, edition: 4, DOI
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Introduction to CytoSurge FluidFM module
Year: 2021, type: Workshop
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Introduction to Hysitron BioSoft Nanoindenter
Year: 2021, type: Workshop
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Introduction to JPK NanoWizard 4 AFM microscope
Year: 2021, type: Workshop
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Investigation of the structure and dynamics of gallium binding to high-affinity peptides elucidated by multi-scale simulation, quantum chemistry, NMR and ITC dagger
Physical Chemistry Chemical Physics, year: 2021, volume: 23, edition: 14, DOI
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Knihovna cílených hmotnostně-spektrometrických assays pro proteotypovou klasifikaci trojitě negativních nádorů prsu získaná pomocí timsTOF Pro
Year: 2021, type: Conference abstract
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Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles
Microchimica Acta, year: 2021, volume: 188, edition: 5, DOI