Project information
Interaction of biopolymers with ligands and detection of their conformation changes at interfaces by electrochemical and optical methods.
- Project Identification
- IAA4004404
- Project Period
- 1/2004 - 12/2006
- Investor / Pogramme / Project type
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Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Václav Holý, CSc.
- prof. RNDr. Vladimír Vetterl, DrSc.
- Keywords
- adsorption of bases and nucleosides; DNA adsorption; 2-D condensation; electrode double layer; electrochemical impedance spectroscopy; EIS; difractive optical element; DOE; self-assembled layers of bases and nucleosides; SAM; DNA biosensor;
Self-assembled layers (SAM) of nucleic acid bases, nucleosides, nucleotides, alkylthiols, quarternary ammonium salts and phospholipids adsorbed at the surfaces of mercury, graphite, silicon and other conductive and semiconductive surfaces will be studied by electrochemical impedance spectroscopy, Raman spectroscopy, diffractive optical element (DOE) sensor, X-ray reflection and AFM microscopy. Recently the DOE sensor was applied in surface structural characterisation of the carbon electrodes (glassy carbon, pyrolytic graphite) modified by mercury layers. The interactions between adsorbed bases can result in their two-dimensional (2-D) condensation and formation of a compact layer. The effect of structural morphology of the chemical modified surfaces on the formation and properties of the SAM layers of the nucleic acid and phospholipids will be studied. The results will contribute to the development of DNA biosensor.
Publications
Total number of publications: 17
2005
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Electrochemical properties of nucleic acid components.
Electrochemistry of nucleic acids and proteins. Towards electrochemical sensors for genomics and proteomics., edition: Vyd. 1., year: 2005, number of pages: 52 s.
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Self-assembled layers of nucleic acid bases and nucleosides at the interfaces and the origin of life.
European Biophysics Journal, year: 2005, volume: 34/2005, edition: 6
2004
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Application of thin film mercury electrodes and solid amalgam electrodes in electrochemical analysis of the nucleic acids components: detection of the two-dimensional phase transients of adenosine.
Bioelectrochemistry, year: 2004, volume: 2004, edition: 63
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Comparison of the low-frequency magnetic field effects on bacteria Escherichia coli, Leclercia adecarboxylata and Staphylococcus aureus
Bioelectrochemistry, year: 2004, volume: 2004, edition: 63
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Detection of phase transients in two-dimensional adlayers of adenosine at the solid amalgam electrode surfaces
Journal of Electroanalytical Chemistry, year: 2004, volume: 2004, edition: 568
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Effect of pH and substituents on the two-dimensional condensation of cytosine
Book of Abstracts, 10th International conference of electrified interfaces-on non-traditional methods, year: 2004
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Effect of the low-frequency magnetic fields on the living organisms
Biological effects of EMFs - 3rd international workshop, year: 2004