Project information
Nové strategie úpravy vzorků pro desorpční hmotnostní spektrometrii
- Project Identification
- GAP206/12/0538
- Project Period
- 1/2012 - 12/2014
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
Navrhujeme vyuzt multidetekcn platformu vyvinutou v predchozm projektu v metalomice, pro analyzu
vybranych biologickych, klinickych, potravinarskych a environmentalnch vzorku. Provedeme dukladnou
charakterizaci puvodnch technik prpravy a zpracovan vzorku pro prvkovou analyzu a speciaci pomoc
ICP MS, termalnho odparovan pomoc laserove diody (LDTV) a laserove desorpce za ucasti substratu
(SALD), a vysetrme moznosti jejich aplikac pro jednoduchou roztokovou analyzu i pro speciaci v kombi-
naci s kolonovymi a planarnmi separacnmi technikami. Sestrojme cenove prijatelne zarzen pro LDTV
jako alternativu nebulizeru pro prvkovou roztokovou analyzu. Vzorky budou zahrnovat metalothioneiny,
fosforylovane proteiny i anorganicke slouceniny obsahujc kovy a komplexy s bioligandy. Kombinace kapi-
larn elektroforezy s hmotnostn spektrometri SALD ICP a MALDI, techniky s overenou, vysokou separacn
ucinnost a citlivost, budou pouzity v nejnarocnejsch aplikacch. Demonstrujeme vyhody uvedenych technik
pro archivovan a prepravu vzorku, jednoduchou kalibraci a rychlost analyzy.
Publications
Total number of publications: 52
2014
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Diode laser thermal vaporisation inductively coupled plasma mass spectrometry of cobalamins
Year: 2014, type: Conference abstract
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Diode laser thermal vaporization - novel sample introduction technique for ICP MS
CECE 2014: 11th International Interdisciplinary Meeting on Bioanalysis, year: 2014
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Diode laser thermal vaporization ICP MS with a simple tubular cell for determination of lead and cadmium in whole blood
Journal of Analytical Atomic Spectrometry, year: 2014, volume: 29, edition: 9, DOI
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Liquid chromatography with substrate-assisted laser desorption/ionization mass spectrometry for determination of sterols
Year: 2014, type: Appeared in Conference without Proceedings
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MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics
Analytical Chemistry, year: 2014, volume: 86, edition: 1, DOI
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New detection approaches for microscale separations based on laser desorption mass spectrometry
Year: 2014, type: Appeared in Conference without Proceedings
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Novel coupling of separations with laser desorption elemental and molecular mass spectrometry
Year: 2014, type: Appeared in Conference without Proceedings
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Off-line CE-MALDI MS/SALD ICP MS coupling: what can we acquire from a single separation run?
Year: 2014, type: Appeared in Conference without Proceedings
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Off-line spojení separací v kapalné fázi a hmotnostní spektrometrie
Year: 2014, type:
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Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam
Analytical Chemistry, year: 2014, volume: 86, edition: 2, DOI