Off-line CE-MALDI MS/SALD ICP MS coupling: what can we acquire from a single separation run?

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Authors	TOMALOVÁ Iva POLANSKÝ Ondřej FOLTYNOVÁ Pavla KANICKÝ Viktor PREISLER Jan
Year of publication	2014
Type	Appeared in Conference without Proceedings
MU Faculty or unit	Central European Institute of Technology
Citation
Description	Single run of CE coupled to MALDI MS and SALD-ICP MS provides highly complementary information about present biomolecules and metals. Moreover, SALD-ICP MS allows reliable metal quantitation, which was performed using a calibration curve approach. Both protein apoforms and protein-metal complexes can be detected using MALDI MS, depending on the applied MALDI matrix and the pH of the matrix solution. MALDI mass spectra of metal-protein complexes are compared with corresponding ESI mass spectra.
Related projects:	CEITEC - Central European Institute of Technology Nové strategie úpravy vzorků pro desorpční hmotnostní spektrometrii Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci