Project information
Threedimensional analysis of cell nuclei using image cytometry
- Project Identification
- GP204/03/D034
- Project Period
- 9/2003 - 8/2006
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Postdoctoral projects
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- high-resolution image cytometry, FISH, 3D image analysis
An instrument called high-resolution image cytometer provides precise
measurements (with resolution comparable to confocal microscopy) on a large number of FISH stained cell nuclei (1000-10000 objects). The
cytometer can be used for both basic biological research (genetic material
structure studies) and clinical applications. The cytometer was developed
in our laboratory and is unique in the world. This excellent instrument
has, unfortunately, one big limitation - it cannot perform full 3D
studies. The aim of this project is to improve the high-resolution image
cytometer so that it will provide complete 3D analysis of a large number
of cell nuclei. Capabilities of image cytometer are determined by the
image analysis subsystem, which will be improved. After the extension the
computer will be able to construct a 3D model of a cell nucleus in its
memory and therefore new set of 3D measurements will be possible, e.g. the
computation of point-to-surface distances, volume or area of nuclei or
chromozomes.
Results
Publications
Total number of publications: 7
2006
-
Fast and Robust Segmentation of Low Contrast Biomedical Images
Visualization Imaging and Image Processing, year: 2006
-
Fast Point-Based 3D Alignment of Live Cells
IEEE Transactions on Image Processing, year: 2006, volume: 15, edition: 8
-
Improved 3D Reconstruction of Interphase Chromosomes Based on Nonlinear Diffusion Filtering
Proceedings of PDE-Based Image Processing and Related Inverse Problems, year: 2006
2004
-
Fast Marching 3D Reconstruction of Interphase Chromosomes
Computer Vision and Mathematical Methods in Medical and Biomedical Image Analysis, year: 2004
-
High-Resolution Cytometry Network Project: Client/Server System for 3D Optical Microscope Data Storage and Analysis
2nd International Symposium on 3D Data Processing, Visualization, and Transmission, year: 2004
-
Image analysis of the movement of sub-cellular structures
Biophysics of the Genome, year: 2004
-
Rapid Image Acquisition of Living Cells and its Limits
Biophysics of the Genome, year: 2004