Efficiency of PCR-based methods in discriminating Bifidobacterium longum ssp. longum and Bifidobacterium longum ssp. infantis strains of human origin

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Authors	ŠRŮTKOVÁ Dagmar ŠPANOVÁ Alena ŠPANO Miroslav DRÁB Vladimír SCHWARZER Martin KOZÁKOVÁ Hana RITTICH Bohuslav
Year of publication	2011
Type	Article in Periodical
Magazine / Source	Journal of Microbiological Methods
MU Faculty or unit	Faculty of Science
Citation
Doi	http://dx.doi.org/10.1016/j.mimet.2011.06.014
Field	Genetics and molecular biology
Keywords	Bifidobacterium longum; PCR; ARDR; RAPD; rep-PCR
Description	Four molecular-biological methods (PCRs, random amplified polymorphic DNA (RAPD) method using 5 primers, repetitive sequence-based (rep)-PCR with BOXA1R and (GTG)5 primers and amplified ribosomal DNA restriction analysis (ARDRA)) and biochemical analysis were compared for the classification of B. longum strains isolated from the faeces of breast-fed healthy infants (25), healthy adults (3) and 2 collection strains. These methods together with arabionose/ melezitose fermentation analysis clearly differentiated only the collection strains into B. longum ssp. longum and B. longum ssp. infantis at the subspecies level. On the other hand, ARDRA analysis differentiated the strains into the B. longum/infantis subspecies using the cleavage analysis of genus-specific amplicon with just one enzyme, Sau3AI. Moreover, we propose a classification of the collection strains into the subspecies level: B. longum CCM 3764 as B. longum ssp. longum CCM 3764, B. longum ATCC 17930 as B. longum ssp. infantis ATCC 17930.
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