Magnetic Microspheres for DNA Diagnostics and DNA Delivery

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Authors

RITTICH Bohuslav ŠPANOVÁ Alena TRACHTOVÁ Štěpánka HORÁK Daniel

Year of publication 2010
Type Article in Proceedings
Conference Sixth International Conference on Materials Structure and Micromechanics of Fracture
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; poly(N-isopropylacrylamide); enzyme immobilization; DNA isolation; PCR
Description Magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) [P(HEMA-EDMA))], poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [P(HEMA-GMA)], and poly(glycidyl methacrylate) [PGMA] microspheres were prepared by a single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in molecular diagnostic was demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic and iminodiacetic acid immobilised on magnetic PGMA and P(HEMA-EDMA) microspheres, respectively, were used as catalysts of plasmid DNA cleavage. Monodisperse poly(N-isopropylacrylamide) microparticles were prepared by two methods, namely, ammonium persulfate-initiated precipitation polymerization in water and 2,2'-azobisisobutyronitrile-initiated and poly(hydrogenated isoprene-block-styrene)-stabilized dispersion polymerization in toluene/heptane. Microspheres were applied for reversible adsorption of DNA at different temperatures.
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