Identifikace laktobacilů izolovaných z potravin pomocí PCR a MALDI-TOF MS

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Title in English	Identification of lactobacilli isolated from food by PCR and MALDITOF MS
Authors	DUŠKOVÁ Marta ŠEDO Ondrej ZDRÁHAL Zbyněk KARPÍŠKOVÁ Renata
Year of publication	2010
Type	Article in Proceedings
Conference	XII. Konference mladých vědeckých pracovníků s mezinárodní účastí
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Description	The aim of this study was to compare two methods for the species identification. 175 strains of lactobacilli isolated from milk and meat products (n = 139) were identified by genotypic and chemotaxonomic methods: the polymerase chain reaction (PCR) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Using the PCR method strains were differentiated into 10 species and into 16 species by MALDI-TOF MS. The total agreement of both methods has been observed in five species (L. curvatus, L. delbrueckii, L. johnsonii, L. plantarum a L. sakei).
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