Capillary Electromigration Separation of Proteins and Microorganisms Dynamically Modified by Chromophoric Nonionogenic Surfactant

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Authors

HORKÁ Marie RŮŽIČKA Filip HOLÁ Veronika KAHLE Vladislav MORAVCOVÁ Dana ŠLAIS Karel

Year of publication 2009
Type Article in Periodical
Magazine / Source Analytical Chemistry
MU Faculty or unit

Faculty of Medicine

Citation
Field Analytic chemistry
Keywords Capillary Electromigration Techniques; Proteins; Microorganisms; Dynamic Modification; Chromophoric Non-ionogenic Surfactant
Description Chromophoric non-ionogenic surfactant poly(ethylene glycol) 3-(2-hydroxy-5-n-octylphenylazo)-benzoate, HOPAB, has been prepared and used as a buffer additive for a dynamic modification of proteins and/or microorganisms including Escherichia coli, Staphylococcus epidermidis (biofilm positive and negative), the strains of yeast cells, Candida albicans, Candida parapsilosis (biofilm positive and negative) during a capillary electrophoresis and a capillary isoelectric focusing with UV detection at 326 nm. Minimum detectable amount has been assessed lower than picograms of proteins and lower than a hundred cells injected into a separation capillary. The introduced labeling method facilitates CIEF separation of microorganisms from the clinical sample of the infected urine at their clinically important level in the pH gradient pH range 2-5 and their subsequent cultivation.
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