Identification of bacterial strains of species Lactococcus lactis in hard cheese using PCR

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Authors

ŠPANOVÁ Alena HERZOGOVÁ Jitka RITTICH Bohuslav

Year of publication 2008
Type Article in Proceedings
Conference 4th Meeting on Chemistry and Life, Chemické listy,102 (2008) No15
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords cheese; Lactococcus lactis; P(HEMA-co-EDMA); PCR;
Description PCR-ready DNA was isolated from different type of cheese using magnetic microspheres P(HEMA-co-EDMA) containing carboxyl groups in the presence of high concentrations of PEG 6000 and NaCl. The quality of extracted DNA was checked by PCR amplification. Species-specific PCR primers PALA4 and PALA14 (targeted on acm A gene encoding N-acetylmuramidase specific to Lactococcus lactis, 1131 bp long PCR products ) were used for the identification of Lactococcus lactis species with success.
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