Separace amplikonů specifických pro rod Lactobacillus s využitím magnetického nosiče

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Title in English Separation of amplicons specific for genus Lactobacillus by magnetic carrier
Authors

JANOSZOWSKÁ Dagmar ŠPANOVÁ Alena RITTICH Bohuslav HORÁK Daniel

Year of publication 2008
Type Article in Proceedings
Conference XII. Setkání biochemiků a molekulárních biologů
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Lactobacillus; DNA isolation; magnetic microspheres; streptavidin
Description DNA isolated from bacterial cells of genus Lactobacillus was amplified using genus specific primers LbLMA1 a R-16-1 (Dubernet a spol., 2002). Primer R16-1 was biotinyled. Biotinyled PCR products were separated using magnetic carrier with immobilised streptavidin. The elution of PCR product from carrier was tested.
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