Identifikace a studium funkce mikroRNA s prediktivním a prognostickým významem u pacientů s kolorektálním karcinomem
| Title in English | Identification and functional studies of microRNAs with predictive and prognostic significance in colorectal cancer patients |
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| Authors | |
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | Edukační sborník. XXXII. Brněnské onkologické dny s XXII. Konferencí pro sestry a laboranty. (ISBN: 978-9780-86793-11-5) |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.mou.cz/mou/upload/Rubriky/BOD/Sbornik_BOD2008.pdf |
| Field | Oncology and hematology |
| Keywords | microRNA; colorectal cancer; profiling; p53; apoptosis |
| Description | MicroRNAs (miRNAs) are a large group of short non-coding RNA post-transcriptionally regulate gene expression. Based on the ability miRNAs regulate translation of oncogenes and tumor suppressor genes, can assume the functional involved in the carcinogenesis process. In the research of cancer of the colon and rectum have been published work that defines the expression profiles of miRNA distinguish tumor tissue from a healthy intestinal mucosa or the ability of selected miRNA function have tumor suppressor or oncogene. In our study, using Real-Time PCR followed the relationship of selected miRNAs to clinico-pathological characteristics of colorectal cancer (CRC) on a set of 29 patients. Highest levels in tumor tissue compared to normal intestinal epithelium showed miR-21 (p = 0.0001), which we also associate with positive lymph nodes (p = 0.027) and the development of distant metastases (p = 0.009). To clarify the function of miR-21 in the pathogenesis of CRC, we conducted an in vitro study on colorectal cancer cells SW837 and DLD1. To this end, we have the transient cells transfekovali 2-O-methyl oligonucleotides, anti-miRNA-21, leading to low levels reprodukovatelnému miR-21 to 12% -15% compared to expression in control cells, which was transfected into 2-Ome- EGFP. For the normalization of the data we used a small nuclear RNA U6B (RNU6B), whose expression was transfection in no case affected. Transfection of anti-miRNA-21 resulted in approximately 30% decrease in the proliferation of both tested lines in the measurement of MTT test. Currently we are testing the impact of anti-miRNA-21 in the sensitization of tumor cells to chemotherapeutic agents Panel (5-fluorouracil, irinotecan, oxaliplatin), its effect on cell cycle by flow cytometry and invasive |