A simple procedure for isolation of PCR-ready bacterial DNA

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Authors	ŠPANOVÁ Alena RITTICH Bohuslav KŘÍŽOVÁ Jana NĚMCOVÁ Eva KORDAČOVÁ Lucie
Year of publication	2007
Type	Article in Proceedings
Conference	Ninth International Symposium on Advances in Extraction Technologies
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	DNA; isolation; salting-out; NaCl
Description	PCR-ready DNA was isolated using salting-out procedure from crud cell lysates. The evaluated method is suitable for DNA isolation from pure cultures of bacterial cells. The reproducibility of RAPD and rep-PCR paterns was comparable with phenol extraction procedure.
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