Ribotyping of lactobacilli isolated from spoiled beer

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Authors

YANSANJAV Altanzaya ŠVEC Pavel SEDLÁČEK Ivo HOLLEROVÁ Ida NĚMEC Miroslav

Year of publication 2003
Type Article in Periodical
Magazine / Source FEMS Microbiology Letters
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords Lactobacillus; ribotyping; beer
Description Twenty-nine Lactobacillus strains contaminating beers in different Czech breweries as well as representative type strains obtained from the Czech Collection of Microorganisms were characterized using ribotyping with EcoRI and a probe made complementary to 16S and 23S rRNA genes. Biochemical test results assigned the 29 strains to the species L. brevis, L. plantarum, L. buchneri and L. paracasei subsp. paracasei. Ribotyping separated L. brevis, L. plantarum and L. paracasei subsp. paracasei strains into species-specific ribogroups in full correspondence with biotyping; L. buchneri strains were split into two ribogroups. Characteristic band patterns for each species and even typical bands of certain sizes were observed.
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