Elektrochemická detekce interakcí chinoxalinových a antracyklinových antibiotik s molekulami DNA.

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Title in English	Electrochemical detection of the interactions of chinoxaline and antracykline antibiotics with DNA
Authors	HASOŇ Stanislav DVOŘÁK Jakub JELEN František VETTERL Vladimír
Year of publication	2001
Type	Article in Proceedings
Conference	Book of Abstracts. XXIV.dny lékařské biofyziky
MU Faculty or unit	Faculty of Science
Citation
Field	Biophysics
Keywords	DNA-echinomycin interactions; electrochemical impedance spectroscopy; mercury film electrodes; differential capacitance of the electrode double layer;
Description	The dependence of the impedance Z and/or differential capacitance C of the electrode double layer on electrode potential E and on frequency in solutions of echinomycin with single stranded (ss) and double-helical (double stranded, ds) DNA was measured. Echinomycin gives a pseudocapacitance redox peak on the C-E curves at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. The capacitance measurements can thus distinguish with the aid of bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the electrode surface. This promise to use impedance measurements for detection of dsDNA in hybridization reactins.
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