Dvourozměrná elektroforéza v proteomice: Principy a aplikace

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Title in English Two-dimensional Electrophoresis in Proteomics: Principles and Applications
Authors

BOUCHAL Pavel KUČERA Igor

Year of publication 2003
Type Article in Periodical
Magazine / Source Chemické listy
MU Faculty or unit

Faculty of Science

Citation
Web Publikace v pdf formátu
Field Biochemistry
Keywords Proteom; Proteomika; Dvourozměrná elektroforéza; Hmotnostní spektrometrie; Analýza obrazu; Databáze
Description Proteomics, a new branch of science related to genomics, involves complex analysis of proteins of cells, tissues or biological fluids. Proteomics is today based on two-dimensional electrophoresis, the crucial technology in making protein maps. It consists of special procedure for preparation of sample, sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel visualization. Two-dimensional separations of complex protein mixtures in gels are compared, evaluated by image analysis and protein maps are constructed. Individual proteins on the maps can be characterized by various methods, most frequently by mass spectrometry, and identified in databases using the information on their structure. Proteomic methods find useful applications in comparing complex protein composition in biology, especially in microbiology and medicine, where they can contribute to revealing new relations between concentrations of proteins and their physiologial functions.
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