Stanovení azidothymidinu v lidské moči, krevním séru a krvi

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Title in English	Determination of azidothymidine in human urine, serum and whole blood
Authors	VACEK Jan ANDRYSÍK Zdeněk TRNKOVÁ Libuše ADAM Vojtěch KIZEK René
Year of publication	2002
Type	Article in Proceedings
Conference	Sborník z VI. pracovního setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation
Field	Electrochemistry
Keywords	AZT;Electrochemical methods;Electroanalysis;Reduction of azides;Detection limit of AZT
Description	Azidothymidine (AZT), antiviral drug, was studied in human body liquids(urea,serum and whole blood)by electrochemical methods in connection with hanging mercury electrode(HMDE). Reduction signals of AZT (~1.1 V) obtained using cyclic (CV), differential pulse (DPV, square wave(SWV)and chronopotentiometric stripping analysis (CPSA)were compared.The most suitable method for the AZT determination was SWV where the optimalization of experimental conditions was made. The detection limit AZT in phosphate buffer was 500 pM. It was found that the square wave voltammetry is very fast and chief method for quantification of AZT in body humour. It is able to study the metabolism of this drug and farmacological monitoring of AZT in the case of HIV positive pacients.
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