Metody izolace vybraných proteinů hlíz brambor

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Title in English Methods for isolating proteins of potato tubers
Authors

BÁRTA Jan BÁRTOVÁ Veronika ZDRÁHAL Zbyněk BRABCOVÁ Adéla KAMENOVÁ Andrea DIVIŠ Jiří

Year of publication 2013
MU Faculty or unit

Central European Institute of Technology

Description The basic element of the separation process is a two step procedure based on ion exchange and affinity chromatography. For subsequent fractionation a column with ceramic media and gradient elution via FPLC is used. The aim of the methodology is the determination of two fundamental biological activities of proteins tubers - lipidacylhydrolase patatin activity and activities of protease inhibitors.
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