Study of ergosterol signal pathway
| Autoři | |
|---|---|
| Rok publikování | 2011 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Ergosterol principal compound of the fungal plasma membrane belongs to the group of elicitors called non-specific elicitors whose not differ in their effects on different cultivars within a plant species. It triggers a defence reaction in tobacco and tomato cells manifested by active oxygen species synthesis and changes of ion fluxes. Further production of the phytoalexin capsidiol and pathogenesis related proteins expression was observed. Even though some steps of signalling pathway including phospholipase A2 protein kinase C and intracellular calcium were proposed up today activation mechanism was not elucidate. But it is assumed role of some specific binding partner or lipid rafts. Here we have attempted to elucidate in more details of the signal pathway elicited by ergosterol. Therefore we focused on study of calcium signalling by using fluorescence confocal microscopy and specific inhibitors. Especially, we tested treatment of ergosterol on vacuoles in comparison with vacuole calcium release activators. Moreover, we tried to localise site of ergosterol recognition. We detected that elicitation by ergosterol increases the concentration of cytosolic calcium mainly from internal sources but direct activation of calcium release from vacuole by ergosterol didnt determined. |
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