High resolution positioning of intron ends on the nucleosomes
| Autoři | |
|---|---|
| Rok publikování | 2011 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Gene |
| Fakulta / Pracoviště MU | |
| Citace | |
| Doi | http://dx.doi.org/10.1016/j.gene.2011.08.022 |
| Obor | Genetika a molekulární biologie |
| Klíčová slova | AG-ends of introns; Gene splicing; GT-ends of introns; Nucleosome mapping; Protection of splice junctions; Rotational positioning |
| Popis | High resolution sequence-directed nucleosome mapping is applied to 36,000 sequences containing splice junctions, from five different species.Moreover, the orientation of guanine residues at the GT- and AG-ends of introns within the nucleosomes is such that the guanines are positioned nearest to the surface of histone octamers, 3 and 4 bases upstream from the local DNA pseudo-dyads passing through minor grooves oriented outwards. Since the guanine residues are the most vulnerable to spontaneous damage within the cell (primarily, depurination and oxidation) such positioning of the splice junctions minimizes the damage that is caused by free radicals and highly reactive metabolites. |
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