Hypomethylating drugs efficiently decrease cytosine methylationin telomeric DNA and activate telomerase without affectingtelomere lengths in tobacco cells

Logo poskytovatele
Logo poskytovatele
Logo poskytovatele

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
Autoři

MAJEROVÁ Eva FOJTOVÁ Miloslava MOZGOVÁ Iva BITTOVÁ Miroslava FAJKUS Jiří

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj Plant Molecular Biology
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
Doi http://dx.doi.org/10.1007/s11103-011-9816-7
Obor Genetika a molekulární biologie
Klíčová slova Nicotiana tabacum; Cell culture; Telomere; Hypomethylating agents; Zebularine; DNA methylation
Popis Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we show that a considerable fraction of cytosines is methylated not only in subtelomeric, but also in telomeric DNA of tobacco BY-2 cells. Drug-induced hypomethylation (demonstrated at subtelomeric, telomeric, and global DNA levels) results in activation of telomerase. However, in contrast to mouse cells, the decrease in 5-methylcytosine levels and upregulation of telomerase do not result in any changes of telomere lengths. These results demonstrate the involvement of epigenetic mechanisms in the multilevel process of regulation of telomerase activity in plant cells and, at the same time, they indicate that changes in telomerase activity can be overridden by other factors governing telomere length stability.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info