ABC transporters affect the detection of intracellular oxidants by fluorescent probes.

• Autoři: PROCHÁZKOVÁ Jiřina; KUBALA Lukáš; KOTASOVÁ Hana; GUDERNOVÁ Iva; ŠRÁMKOVÁ Zuzana; PEKAROVÁ Michaela; SARKADI Balazs; PACHERNÍK Jiří
• Rok publikování: 2011
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Free Radical Res.
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• Doi: http://dx.doi.org/10.3109/10715762.2011.579120
• Obor: Fyziologie
• Klíčová slova: Fluorescence; redox status; mitochondria; reactive oxygen species (ROS)
• Popis: Intracellular production of reactive oxygen species (ROS) plays an important role in the control of cell physiology. For the assessment of intracellular ROS production, a plethora of fluorescent probes is commonly used. Interestingly, chemical structures of these probes imply they could be substrates of plasma membrane efflux pumps, called ABC transporters. This study tested whether the determination of intracellular ROS production and mitochondrial membrane potential by selected fluorescent probes is modulated by the expression and activity of ABC transporters.

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