Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Fakultu informatiky. Oficiální stránka publikace je na webu muni.cz.

Autoři	ŠUSTÁČKOVÁ Gabriela KOZUBEK Stanislav STIXOVÁ Lenka LEGARTOVÁ Soňa MATULA Pavel ORLOVA Darya Yurevna BÁRTOVÁ Eva
Rok publikování	2012
Druh	Článek v odborném periodiku
Časopis / Zdroj	Journal of cellular physiology
Fakulta / Pracoviště MU	Fakulta informatiky
Citace
www	http://onlinelibrary.wiley.com/doi/10.1002/jcp.22912/suppinfo
Doi	http://dx.doi.org/10.1002/jcp.22912
Obor	Genetika a molekulární biologie
Klíčová slova	Polycomb body BMI1 protein arrangement confocal microscopy
Popis	Polycomb group (PcG) proteins, organized into Polycomb bodies, are important regulatory components of epigenetic processes involved in the heritable transcriptional repression of target genes. Here, we asked whether acetylation can influence the nuclear arrangement and function of the BMI1 protein, a core component of the Polycomb group complex, PRC1. We used time-lapse confocal microscopy, micro-irradiation by UV laser (355 nm) and GFP technology to study the dynamics and function of the BMI1 protein. Our data indicate that the dynamics of recognition of UV-damaged chromatin, and the nuclear arrangement of BMI1 protein can be influenced by acetylation and occur as an early event prior to the recruitment of HPb to UV-irradiated chromatin.
Související projekty:	Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii