Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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HORÁK Daniel MATULKA Kamil HLÍDKOVÁ Helena LAPČÍKOVÁ Monika BENEŠ Milan J. JAROŠ Josef HAMPL Aleš DVOŘÁK Petr

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Biomedical Materials Reseach Part B: Applied Biomaterials
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1002/jbm.b.31832
Obor Genetika a molekulární biologie
Klíčová slova poly(NN-diethylacrylamide); porosity; YIGSR; peptide; embryonic stem cells; hydrogel
Popis Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,Ndiethylacrylamide (DEAAm), N,N0-methylenebisacrylamide and methacrylic acid in the presence of (NH4)2SO4 or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH2, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH2 pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSRNH2- modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes.
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