Dexamethasone and Insulin Affecting SFTPA1 and SFTPA2 mRNA Levels

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RUČKA Zdeněk TESAŘOVÁ Lenka POTĚŠILOVÁ Michaela STEJSKAL Stanislav KRONTORÁD KOUTNÁ Irena VAŇHARA Petr DOLEŽEL Jan

Rok publikování 2010
Druh Článek ve sborníku
Konference Advances in Molecular and Cancer Biology, 2010
Fakulta / Pracoviště MU

Fakulta informatiky

Citace
Obor Genetika a molekulární biologie
Klíčová slova Lung surfactant; Insulin; Dexamethasone
Popis Lung surfactants primary function is to lower surface tension and therefore it protects alveoli from collapsing. It consists of phospholipids and four proteins. These proteins stabilize the surfactant layer (SFTPB and SFTPC) and modulate immunological response to various pathogens (SFTPA, SFTPD), which is the secondary role of lung surfactant. Low levels of surfactant proteins lead to severe diseases. Dexamethasone and insulin were applied on H441 cell line. This cell line resembles Clara cells and therefore serves as a model of lung surfactant producing cells. SFTPA1 (and its analog SFTPA2) were not affected by insulin (2500 ng/mL), but dexamethasone treatment (10-7 M) for 48 hours resulted in 50% decrease in their mRNA production. Surprisingly, combination of dexamethasone and insulin resulted in 90% decrease.
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