Directional motion of foreign plasmid DNA to nuclear HP1 foci

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Publikace nespadá pod Fakultu sportovních studií, ale pod Fakultu informatiky. Oficiální stránka publikace je na webu muni.cz.
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ONDŘEJ Vladan KOZUBEK Stanislav LUKÁŠOVÁ Emilie FALK Martin MATULA Pavel MATULA Petr KOZUBEK Michal

Rok publikování 2006
Druh Článek v odborném periodiku
Časopis / Zdroj Chromosome Research
Fakulta / Pracoviště MU

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Citace
Obor Genetika a molekulární biologie
Klíčová slova heterochromatin; HP1 protein; nuclear compartments; plasmid; transfection
Popis Movement of labelled plasmid DNA relative to heterochromatin foci in nuclei, visualized with HP1-GFP, was studied using live-cell imaging and object tracking. In addition to Brownian motion of plasmid DNA we found a pronounced, non-random movement of plasmid DNA towards the nearest HP1 focus, while time-lapse microscopy showed that HP1 foci are relatively immobile and positionally stable. The movement of plasmid DNA was much faster than that of the HP1 foci. Contact of transgene DNA with an HP1 focus usually resulted in cessation of the directional motion. Moreover, the motion of plasmid DNA inside the heterochromatin compartment was more restricted (limited to 0.25 mu m) than when the plasmid DNA was outside heterochromatin (R = 0.7 mu m). Three days after transfection most of the foreign labelled DNA colocalized with centromeric heterochromatin.
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