Comparison of five currently used DC maturation strategies and their influence on DC characteristics

Varování

Publikace nespadá pod Fakultu sportovních studií, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	SKÁLOVÁ Kateřina MOLLOVÁ Klára BUREŠOVÁ Ivana MICHÁLEK Jaroslav
Rok publikování	2010
Druh	Konferenční abstrakty
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Popis	As antigen-presenting cells, dendritic cells (DC) are able to present tumor antigens and effectively stimulate both helper and cytotoxic T-cell response targeted against a tumor. That makes them a suitable source for in vitro cancer vaccines production. Increasing knowledge of DC biology leads to improved methods of their in vitro preparation, but there is to date no consensus specifying optimal conditions for that. It has been demonstrated that DCs derived from monocytic precursors matured in different conditions differ in their characteristics and functional adjustment. We compared five previously described cytokine combinations, currently used for DC maturation, and their effect on following DC characteristics: morphology, surface markers, cytokine production profiles, migratory capacity, capability of T-cell activation and cytotoxicity induction.
Související projekty:	Bezpečná biotechnologie alotransplatovaných hematopoetických kmenových buněk u vybraných solidních a hematologických nádorů jako adoptivní imunoterapie